Jantje Ngangi, Emma Mauren Moko, Dino Rahardiyan
The necessity for enzyme utilization in various industrial applications and reducing production cost for a more economical process compelled this research to seek for an alternative source of endemic enzyme for various sources cellulotic enzymes. The termites hindgut is known as a fermentor chamber of its own containing bacterial isolates with the potential to induce cellulosic substrates. Series of research have been conducted to obtain cellulolytic potential bacterial isolates characterized biochemically and molecularly. The purpose of this research was to determine the enzyme characters of cellulolytic bacterial from the hindguts of endemic termites of North Sulawesi. Screening results from various bacterial isolates from hindguts of local termites of several regencies from North Sulawesi identified Bacillus cereus from the hindgut of Odontotermes javanicus from South Minahasa (North Sulawesi). This Bacillus cereus isolate had the best cellulolytic potential by exhibiting a cellulolytic index of 1.75 cm on a selective media of CMC, with the best enzyme production on the 6th day with the activity of 0.0054U/ml. © 2019 IOP Publishing Ltd.
Biology Department, Mathematics and Natural Sciences Faculty, Manado State University, Tondano, 95619, Indonesia; Agribusiness Department, Faculty of Agricultural, De la Salle Catholic University, Manado, 95000, Indonesia
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