Preliminary laboratory evaluation of Beauveria bassiana (Bals.) Vuill. for the control of Necrobia rufipes (De Geer) on stored copra

Open

Dantje Tarore, Vivi Bernadeth Montong, Jusuf Manueke, Yermia Semuel Mokosuli, Lucia Cecilia Mandey

2025 Bulletin of Insectology Vol. 78 Article Cited by 0 Quartile

Abstract

This study investigates the efficacy of the entomopathogenic fungus Beauveria bassiana (Bals.) Vuill. (Ascomycota: Hypocreales) in controlling Necrobia rufipes (De Geer) (Coleoptera: Cleridae), a significant pest responsible for economic losses in copra production, aiming to determine the effective concentration required to achieve substantial mortality and reduce copra damage in storage systems. Employing a completely randomized design with five treatments and three replications, the research assessed B. bassiana concentrations (10%, 15%, 20%, 25%, and 30%) on N. rufipes mortality and copra weight loss across pest population sizes ranging from 8 to 24 adults, with mortality recorded weekly over one month and weight loss measured after one month. Data analysis using analysis of variance, followed by a least significant difference test and probit analysis, revealed mortality rates from 14.67% (±2.31%) at 10% concentration to 69.33% (±3.79%) at 30%, with an LC50 of 23.611% (±1.12%), while copra weight loss increased from 1.33 g (±0.15 g) with 8 adults to 6.87 g (±0.42 g) with 21 adults, strongly correlating with pest density. These findings demonstrate that B. bassiana effectively reduces N. rufipes populations, with higher concentrations enhancing control, and the identified LC50 provides a practical application threshold, underscoring the influence of pest population size on copra weight loss and the need for timely intervention. This approach offers a sustainable, biological strategy to protect copra, potentially minimizing economic losses in tropical storage systems. © Alma Mater Studiorum – Università di Bologna.

Affiliations

Faculty of Agriculture, Sam Ratulangi University, Manado, Indonesia; Postgraduate Program, Sam Ratulangi University, Manado, Indonesia; Faculty of Mathematics and Natural Sciences, Manado State University, Manado, Indonesia